Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 23 (1), 175-82

SV40-transformed Simian Cells Support the Replication of Early SV40 Mutants

SV40-transformed Simian Cells Support the Replication of Early SV40 Mutants

Y Gluzman. Cell.

Abstract

CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.

Similar articles

See all similar articles

Cited by 953 PubMed Central articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback