SV40-transformed simian cells support the replication of early SV40 mutants

Cell. 1981 Jan;23(1):175-82. doi: 10.1016/0092-8674(81)90282-8.


CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Cell Transformation, Viral*
  • Chromosome Deletion
  • Defective Viruses / genetics
  • Genes, Viral
  • Mutation
  • Simian virus 40 / genetics*
  • Temperature
  • Virus Replication*