Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein

Proc Natl Acad Sci U S A. 1981 Apr;78(4):2043-7. doi: 10.1073/pnas.78.4.2043.

Abstract

The polypeptide product of the lon (capR) gene was identified and partially purified from bacterial strains homozygous for the capR(+) or capR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR(+) and capR9 proteins bind DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / isolation & purification
  • Carrier Proteins / genetics*
  • Carrier Proteins / isolation & purification
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA, Bacterial / metabolism
  • DNA-Binding Proteins
  • Escherichia coli / genetics*
  • Genes
  • Molecular Weight
  • Mutation

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • DNA