The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit

Eur J Biochem. 1981 Jun 1;116(3):621-9. doi: 10.1111/j.1432-1033.1981.tb05381.x.

Abstract

The combined structural study of proteins and of their corresponding genes utilizing the methods of both protein and nucleotide chemistry greatly accelerates and considerably simplifies both the nucleotide and protein structure determination and, in particular, enhances the reliability of the analysis. This approach has been successfully applied in the primary structure determination of the beta and beta' subunits of Escherichia coli DNA-dependent RNA polymerase and of their structural genes, yielding a continuous nucleotide sequence (4714 base pairs) that embraces the entire rpoB gene, the initial part of the rpoC gene and the intercistronic region, together with the total amino acid sequence of the beta subunit, comprising 1342 residues, and the N-terminal sequence of the beta' subunit (176 residues).

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics*
  • DNA-Directed RNA Polymerases / genetics*
  • Escherichia coli / enzymology*
  • Genes*
  • Macromolecular Substances
  • Plasmids

Substances

  • DNA, Bacterial
  • Macromolecular Substances
  • DNA-Directed RNA Polymerases
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J01678
  • GENBANK/K00449