Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping

J Med Virol. 1981;7(2):119-25. doi: 10.1002/jmv.1890070205.

Abstract

A new human adenovirus has been isolated from patients with keratoconjunctivitis and/or genital infection since 1976. This adenovirus, designed candidate adenovirus 37 (Ad 37) is serologically distinct but related to Ad 10, 13, 19, and 30 (see the accompanying paper by de Jong et al). SDS-Polyacrylamide gel electrophoresis of Ad 37 virion polypeptides showed that this adenovirus is a member of subgroup D. DNA restriction endonuclease analysis of DNA from Ad 37 and related serotypes belonging to subgroup D showed that Ad 37 is a new genome type belonging to subgroup D but clearly distinct from the 20 serotypes classified into this subgroup.

MeSH terms

  • Adenoviruses, Human / analysis*
  • Adenoviruses, Human / classification*
  • Chromosome Mapping
  • DNA Restriction Enzymes / metabolism
  • DNA, Viral / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Keratoconjunctivitis / microbiology*
  • Sodium Dodecyl Sulfate
  • Viral Proteins / analysis*
  • Virion / analysis

Substances

  • DNA, Viral
  • Viral Proteins
  • Sodium Dodecyl Sulfate
  • DNA Restriction Enzymes