DNA methyltransferases affecting the sequence 5'CCGG

Nucleic Acids Res. 1981 Jun 25;9(12):2753-9. doi: 10.1093/nar/9.12.2753.

Abstract

B. subtilis phage SPbeta and Moraxella sp. code for DNA methyltransferases which methylate both cytosines of the sequence 5'CCGG. Experiments using a B. subtilis strain whose DNA is sensitive to HpaII and resistant to MspI degradation, indicated that methylation of the outer C of this sequence provides protection against the restriction enzyme MspI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / enzymology*
  • Bacillus subtilis / genetics
  • Bacteriophages / enzymology*
  • Bacteriophages / genetics
  • Base Sequence
  • DNA (Cytosine-5-)-Methyltransferases / genetics*
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics*
  • Methyltransferases / genetics*
  • Moraxella / enzymology*
  • Moraxella / genetics
  • Substrate Specificity

Substances

  • DNA, Bacterial
  • Methyltransferases
  • DNA (Cytosine-5-)-Methyltransferases
  • DNA Restriction Enzymes