The DNA sequence of the promoter-attenuator of the ilvGEDA operon of Salmonella typhimurium

Nucleic Acids Res. 1981 Jul 24;9(14):3419-32. doi: 10.1093/nar/9.14.3419.

Abstract

The isolation of a lambda gt . ilvGEDA . S.t. hybrid transducing phage has permitted the characterization of the promoter-attenuator region of the ilvGEDA operon of Salmonella typhimurium. In vitro transcription and Southern hybridization indicate that the promoter-attenuator resides on a 400 nucleotide Rsa I restriction fragment. DNA sequence analysis shows only seven base pair differences exist between the DNA sequence of the ilvGEDA promoter-attenuator of S. typhimurium and that previously published for Escherichia coli K12.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics
  • Genetic Code
  • Nucleic Acid Hybridization
  • Operon*
  • Salmonella Phages / genetics
  • Salmonella typhimurium / genetics*
  • Species Specificity
  • Transcription, Genetic
  • Transduction, Genetic

Substances

  • DNA, Bacterial
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J01806
  • GENBANK/M11654