Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene

Nature. 1981 Oct 22;293(5834):620-5. doi: 10.1038/293620a0.

Abstract

By replacing either the eight early or the late genes of SV40 with a cloned copy of the influenza virus haemagglutinin gene we have constructed recombinant viruses which, in infected cells, express large quantities of haemagglutinin. This glycoprotein, over 10(8) molecules of which are produced per cell, is identical in molecular weight to authentic influenza virus haemagglutinin, accumulates at the cell surface and displays haemabsorbing activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular / methods
  • DNA, Recombinant
  • Gene Expression Regulation
  • Genetic Vectors
  • Glycoproteins / genetics
  • Hemagglutinins, Viral / genetics*
  • Influenza A virus / genetics*
  • Membrane Proteins / genetics
  • RNA, Viral / genetics
  • Simian virus 40 / genetics

Substances

  • DNA, Recombinant
  • Glycoproteins
  • Hemagglutinins, Viral
  • Membrane Proteins
  • RNA, Viral