A simple method for the isolation of basolateral plasma membrane vesicles from rat kidney cortex. Enzyme activities and some properties of glucose transport

Biochim Biophys Acta. 1981 Sep 21;647(1):150-4. doi: 10.1016/0005-2736(81)90303-5.

Abstract

A procedure for preparing basolateral membrane vesicles from rat renal cortex was developed by differential centrifugation and Percoll density gradient centrifugation, and the uptake of D-[3H] glucose into these vesicles was studied by a rapid filtration technique. (Na+ + K+)-ATPase, the marker enzyme for basolateral membranes, was enriched 22-fold compared with that found in the homogenate. The rate of D-glucose uptake was almost unaffected by Na+ gradient (no overshoot).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Aminopeptidases / metabolism
  • Animals
  • Biological Transport, Active
  • Cell Fractionation / methods*
  • Cell Membrane / enzymology*
  • Centrifugation, Density Gradient / methods
  • Glucose / metabolism*
  • Kidney Cortex / enzymology*
  • Male
  • Rats
  • Rats, Inbred Strains
  • Sodium-Potassium-Exchanging ATPase / metabolism

Substances

  • Alkaline Phosphatase
  • Aminopeptidases
  • Sodium-Potassium-Exchanging ATPase
  • Glucose