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. 1981 Sep;14(4):289-99.
doi: 10.1016/0378-1119(81)90161-x.

The Plasmid Cloning Vector pBR325 Contains a 482 Base-Pair-Long Inverted Duplication

The Plasmid Cloning Vector pBR325 Contains a 482 Base-Pair-Long Inverted Duplication

P Prentki et al. Gene. .

Abstract

The plasmid pBR325 is a cloning vector constructed in vitro by addition of the chloramphenicol resistance (Cmr) gene of an IS1-flanked transposon to pBR322 (Bolivar, 1978). It is a 5 995 bp plasmid carrying no sequence originating from IS1. DNA-sequence data suggest that its Cmr segment was derived from a Cm transposon longer than Tn9. The plasmid pBR325 carries between the Cmr and Tcr genes a 482 bp sequence which duplicates, in the opposite orientation, a section pf pBR322 located at the end of the tcr gene. The same structure was found in pBR328, a deletion derivative of pBR325 (Soberon et al., 1980). The possible implications of this inverted duplication on cloning experiments are discussed.

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