Glucocorticoids Regulate Expression of Dihydrofolate Reductase cDNA in Mouse Mammary Tumour Virus Chimaeric Plasmids

Nature. 1981 Nov 19;294(5838):228-32. doi: 10.1038/294228a0.


Fusions between the mouse mammary tumour virus long terminal repeat and a mouse dihydrofolate reductase cDNA have been constructed in a SV40 vector. When these plasmids are transferred into recipient cells, the production of dihydrofolate reductase is regulated by glucocorticoid hormones. These results define a hormonally responsive region of the viral genome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Transformation, Viral / drug effects*
  • Cloning, Molecular*
  • DNA, Recombinant / metabolism
  • Glucocorticoids / pharmacology*
  • Mammary Tumor Virus, Mouse / drug effects
  • Mammary Tumor Virus, Mouse / enzymology
  • Mammary Tumor Virus, Mouse / genetics*
  • Mice
  • Plasmids / drug effects*
  • Tetrahydrofolate Dehydrogenase / genetics*


  • DNA, Recombinant
  • Glucocorticoids
  • Tetrahydrofolate Dehydrogenase