Cloned poliovirus complementary DNA is infectious in mammalian cells

Science. 1981 Nov 20;214(4523):916-9. doi: 10.1126/science.6272391.

Abstract

A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • DNA, Recombinant / metabolism
  • DNA, Viral / genetics*
  • Escherichia coli / genetics
  • Genes, Viral*
  • Plasmids
  • Poliovirus / genetics*
  • RNA, Viral / genetics*
  • Transfection

Substances

  • DNA, Recombinant
  • DNA, Viral
  • RNA, Viral
  • DNA Restriction Enzymes