Abstract
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cloning, Molecular*
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DNA Restriction Enzymes
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DNA, Recombinant / metabolism
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DNA, Viral / genetics*
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Escherichia coli / genetics
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Genes, Viral*
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Plasmids
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Poliovirus / genetics*
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RNA, Viral / genetics*
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Transfection
Substances
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DNA, Recombinant
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DNA, Viral
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RNA, Viral
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DNA Restriction Enzymes