Binding and activation properties of angiotensin II in dispersed rat anterior pituitary cells

Endocrinology. 1982 Mar;110(3):1043-5. doi: 10.1210/endo-110-3-1043.

Abstract

The properties of angiotensin II receptors were studied in isolated rat anterior pituitary cells prepared by trypsin digestion. Angiotensin II bound in a time- and temperature-dependent manner to pituitary cells, with Kd of 4.1 x 10(-9) M. The heptapeptide, des-Asp1-angiotensin II, had only one-tenth of the affinity of the octapeptide (Ki = 5.5 x 10(-8) M). These two peptides displayed a similar potency ratio in their ability to stimulate ACTH release from pituitary cells. These results indicate that angiotensin II may play a regulatory role in controlling ACTH secretion from the pituitary gland.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / metabolism*
  • Angiotensin II / pharmacology
  • Animals
  • Binding, Competitive
  • Female
  • In Vitro Techniques
  • Kinetics
  • Pituitary Gland, Anterior / drug effects
  • Pituitary Gland, Anterior / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Angiotensin / metabolism*
  • Receptors, Cell Surface / metabolism*

Substances

  • Receptors, Angiotensin
  • Receptors, Cell Surface
  • Angiotensin II