Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10

Mol Gen Genet. 1981;184(3):430-3. doi: 10.1007/BF00352517.

Abstract

We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular*
  • Coliphages / genetics
  • DNA Restriction Enzymes
  • DNA Transposable Elements*
  • Escherichia coli / genetics*
  • Genes*
  • Mutation
  • Plasmids
  • Species Specificity

Substances

  • DNA Transposable Elements
  • DNA Restriction Enzymes