Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus

Science. 1982 Sep 3;217(4563):937-9. doi: 10.1126/science.6287573.

Abstract

The transforming protein of Kirsten murine sarcoma virus (Ki-MuSV) is a virally encoded 21-kilodalton protein called p21 kis. The sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned Ki-MuSV DNA to induce focal transformation of mouse cells. Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Transformation, Viral
  • Cells, Cultured
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • DNA, Viral / genetics
  • Genes, Viral
  • Kirsten murine sarcoma virus / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mutation
  • Oncogene Protein p21(ras)
  • RNA, Viral / genetics
  • Sarcoma Viruses, Murine / genetics*
  • Viral Proteins / genetics*

Substances

  • DNA, Recombinant
  • DNA, Viral
  • RNA, Viral
  • Viral Proteins
  • DNA Restriction Enzymes
  • Oncogene Protein p21(ras)

Associated data

  • GENBANK/J02228