Cloning and identification of the product of the dnaE gene of Escherichia coli

J Bacteriol. 1982 Oct;152(1):351-6. doi: 10.1128/jb.152.1.351-356.1982.


We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli chromosome was determined. A unique HindIII restriction endonuclease site within the cloned segment of DNA was identified as a site required for expression of the dnaE gene. By using the maxicell plasmid-directed protein synthesizing system, we demonstrated that dnaE codes for the alpha subunit of DNA polymerase III.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosomes, Bacterial
  • Cloning, Molecular*
  • DNA Polymerase III / genetics*
  • DNA Restriction Enzymes
  • DNA-Directed DNA Polymerase / genetics*
  • Deoxyribonuclease HindIII
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Plasmids


  • Bacterial Proteins
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase
  • DNA Restriction Enzymes
  • Deoxyribonuclease HindIII