Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria

J Biol Chem. 1982 Nov 10;257(21):13028-33.


Yeast mitochondria were fractionated into inner membrane, outer membrane, matrix, and intermembrane space. Identity and purity of each fraction were monitored by enzyme assays, dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological detection of characteristic mitochondrial polypeptides. Cytochrome b2 and cytochrome c peroxidase were found to be components of the intermembrane space. The most reliable marker of the outer membrane was a major 29,000-dalton polypeptide component. The availability of submitochondrial fractions provides a basis for studying import of precursor polypeptides into isolated yeast mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Cell Fractionation
  • Cytochrome-c Peroxidase / metabolism*
  • Intracellular Membranes / metabolism
  • Kinetics
  • L-Lactate Dehydrogenase (Cytochrome)
  • L-Lactate Dehydrogenase / metabolism*
  • Mannitol / pharmacology
  • Mitochondria / drug effects
  • Mitochondria / enzymology*
  • Peroxidases / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Subcellular Fractions / enzymology


  • Mannitol
  • L-Lactate Dehydrogenase
  • L-Lactate Dehydrogenase (Cytochrome)
  • Peroxidases
  • Cytochrome-c Peroxidase