The filamentous phage (Ff) as vectors for recombinant DNA--a review

Gene. 1982 Jul-Aug;19(1):1-10. doi: 10.1016/0378-1119(82)90183-4.


Derivatives of filamentous phage, f1, fd, and M13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

MeSH terms

  • Cloning, Molecular*
  • Coliphages / genetics*
  • DNA Restriction Enzymes
  • DNA, Recombinant*
  • Escherichia coli / genetics*
  • Genotype
  • Species Specificity


  • DNA, Recombinant
  • DNA Restriction Enzymes