Molecular analysis of the c-myc locus in normal tissue and in avian leukosis virus-induced lymphomas

J Virol. 1982 Oct;44(1):158-66. doi: 10.1128/JVI.44.1.158-166.1982.

Abstract

We isolated molecular clones of the provirus-host cell junctions (tumor junction fragments) from two avian leukosis virus-induced lymphomas and compared the structures of these clones with a clone of the normal c-myc gene. Restriction mapping and DNA sequencing demonstrated that normal proviral integration events occurred adjacent to c-myc in both tumors, without gross structural alteration of c-myc. The right long terminal repeat of an avian leukosis virus provirus is integrated upstream from the bulk of the c-myc coding sequences and oriented such that transcription can initiate within the long terminal repeat and proceed downstream into c-myc. A comparison of a tumor junction fragment with the v-myc gene showed that there are two regions of v-myc-related sequences (which are probably exons) separated by 1 kilobase of sequences unrelated to v-myc (probably an intron). A DNA sequence analysis of the tumor junction fragments suggested that integration had occurred in exons adjacent to splice donor sites. This suggests that there are additional exons and introns in c-myc. Based on these findings, a model is proposed for the genesis of the tumor-specific RNAs containing viral-5' and c-myc information in avian leukosis virus-induced lymphomas.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Avian Leukosis / genetics
  • Avian Leukosis / microbiology
  • Avian Leukosis Virus / genetics*
  • Base Sequence
  • Cell Transformation, Neoplastic
  • Cell Transformation, Viral
  • Chickens / genetics
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Genes*
  • Genes, Viral*
  • Lymphoma / genetics
  • Lymphoma / microbiology*
  • Recombination, Genetic*
  • Repetitive Sequences, Nucleic Acid

Substances

  • DNA Restriction Enzymes

Associated data

  • GENBANK/J02007
  • GENBANK/J02008