Sequence of the malK Gene in E.coli K12

Nucleic Acids Res. 1982 Nov 25;10(22):7449-58. doi: 10.1093/nar/10.22.7449.

Abstract

We present the sequence of gene malK which encodes a component of the system for maltose transport in E.coli K12. We also determined the position of deletion (S50) which fuses malK to the following gene lamB; the malK-lamB protein hybrid contains all of the malK protein. The mRNA corresponding to the last two thirds of gene malK could form stable stem and loop structures. The malK protein, as deduced from the gene sequence, would include 370 residues and correspond to a molecular weight of 40700. The sequence as well as sequence comparisons with the ndh protein of E.coli are discussed in terms of the location and function of the malK protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Carrier Proteins / genetics*
  • DNA Restriction Enzymes
  • Escherichia coli / genetics*
  • Escherichia coli Proteins*
  • Genes*
  • Genes, Bacterial*
  • Maltose-Binding Proteins
  • Monosaccharide Transport Proteins*
  • RNA, Messenger / genetics
  • Transcription, Genetic

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • Carrier Proteins
  • Escherichia coli Proteins
  • MalK protein, Bacteria
  • MalK protein, E coli
  • Maltose-Binding Proteins
  • Monosaccharide Transport Proteins
  • RNA, Messenger
  • maltose transport system, E coli
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J01639
  • GENBANK/J01648
  • GENBANK/K02117