Evaluation of ciliary movement in tracheal rings to assess immunity against infectious bronchitis virus

Avian Dis. Oct-Dec 1982;26(4):805-15.


Chickens vaccinated at one and 14 days of age with a live vaccine containing the Massachusetts and Connecticut strains of infectious bronchitis virus were protected against challenge with a Massachusetts strain administered at 5 weeks of age. The virus recovery method in which tracheal swabs taken 4 days post-challenge were used to inoculate chicken embryos was compared with the method based on ciliary activity in tracheal explants. The results showed that evaluation of ciliary activity effectively measured immunity to infectious bronchitis virus. Serological results obtained in a microneutralization test using chicken embryo kidney cells correlated with the immune status of the birds as well as with the alpha neutralization results obtained using chicken embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens*
  • Cilia / physiology*
  • Coronaviridae / immunology*
  • Coronaviridae Infections / immunology
  • Coronaviridae Infections / veterinary*
  • Infectious bronchitis virus / immunology*
  • Infectious bronchitis virus / isolation & purification
  • Movement
  • Organ Culture Techniques
  • Poultry Diseases / immunology*
  • Specific Pathogen-Free Organisms
  • Trachea* / microbiology
  • Trachea* / pathology
  • Viral Vaccines / immunology*


  • Viral Vaccines