Identification of a Mr 58 000 glycoprotein subunit of the opiate receptor

FEBS Lett. 1982 Dec 13;150(1):125-8. doi: 10.1016/0014-5793(82)81318-5.

Abstract

A Mr 58 000 subunit of the opiate receptor has been identified using tritiated fentanyl isothiocyanate, a potent opiate alkylating reagent with specificity for the delta-opiate receptor subclass. The subunit is alkylated in the presence of dextrorphan but not levorphanol. The specifically labelled protein was retained on columns of immobilized wheat germ agglutinin and is therefore presumably a glycoprotein. Partial purification of the Mr 58 000 opiate receptor subunit from neuroblastoma X glioma NG108-15 hybrid cell membranes is described.

MeSH terms

  • Animals
  • Fentanyl / analogs & derivatives
  • Glycoproteins / analysis*
  • Hybrid Cells / analysis
  • Indicators and Reagents
  • Isothiocyanates*
  • Macromolecular Substances
  • Molecular Weight
  • Receptors, Opioid / analysis*

Substances

  • Glycoproteins
  • Indicators and Reagents
  • Isothiocyanates
  • Macromolecular Substances
  • Receptors, Opioid
  • fentanyl isothiocyanate
  • Fentanyl