Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis

Nucleic Acids Res. 1983 Mar 11;11(5):1543-53. doi: 10.1093/nar/11.5.1543.

Abstract

Globin mRNA was isolated from immature red blood cells of an adult Xenopus laevis female. mRNA/cDNA hybrids were integrated in the Pst I cleavage site of pBR 322 by G/C tailing, and cloned in Escherichia coli strain HB 101. By restriction site analysis as well as hybridization behaviour we identified two clones coding for minor adult alpha and beta globin chains. Nucleotide sequence analysis and derived amino acid sequences are presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Erythrocyte Aging
  • Erythrocytes / metabolism
  • Female
  • Globins / genetics*
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics*
  • Xenopus

Substances

  • RNA, Messenger
  • Globins
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X01559
  • GENBANK/X01560