Production of rabbit antibody specific for amino-terminal residues of cholecystokinin octapeptide (CCK-8) by selective suppression of cross-reactive antibody response

J Immunol Methods. 1982 Dec 30;55(3):375-87. doi: 10.1016/0022-1759(82)90097-7.


Antibody specific for the amino-terminal region of cholecystokinin octapeptide (CCK-8) was generated in a highly reproducible way in New Zealand white rabbits by a novel immunization procedure which involves immunization with CCK-8 peptide conjugate coupled with keyhole limpet hemocyanin (KLH) and inhibiting cross-reacting antibody formation by treatment of the animals with a potent tolerogenic conjugate of beta-alanyl-tetragastrin and a copolymer of D-glutamic acid and D-lysine (D-GL). The antisera thus produced specifically react with an amino-terminal region of CCK-8 but not with the non-sulfate form of CCK-8, nor with the carboxy-terminal region which shares a cross-reactive determinant among gastrin and cholecystokinin-related peptides (caerulein, CCK-4, CCK-8, CCK-33 and CCK-39). The antisera produced by this method allowed us to measure specifically CCK in extracts from tissue such as duodenum containing gastrin and CCK at comparable levels.

MeSH terms

  • Amino Acids / immunology*
  • Animals
  • Antibody Formation
  • Antibody Specificity*
  • Brain Chemistry
  • Cholecystokinin / immunology*
  • Cross Reactions
  • Duodenum / analysis
  • Gastrins / analysis
  • Gastrins / pharmacology
  • Humans
  • Immune Sera / analysis
  • Immune Sera / pharmacology
  • Immunologic Techniques*
  • Peptide Fragments / immunology*
  • Rabbits
  • Rats
  • Sincalide
  • Swine


  • Amino Acids
  • Gastrins
  • Immune Sera
  • Peptide Fragments
  • minigastrin
  • Cholecystokinin
  • Sincalide