Abstract
Active rat adrenal angiotensin II receptors have been solubilized with a zwitterionic detergent, 3 [(3-cholamidopropyl)-dimethylammonio] -1-propane sulfonate. The solubilized receptors retain a high affinity for angiotensin II (Kd = 1.9 nM) similar to the value observed in adrenal membrane particles (Kd = 1.2 nM). In addition, the binding-inhibition potency of several angiotensin II peptides is maintained unaltered, indicating a fully preserved specificity of the solubilized receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenal Glands / analysis*
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Angiotensin II / analogs & derivatives
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Angiotensin II / metabolism
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Animals
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Binding, Competitive
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Cholic Acids*
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Detergents
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In Vitro Techniques
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Rats
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Receptors, Angiotensin / analysis*
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Receptors, Cell Surface / analysis*
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Solubility
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Time Factors
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Tissue Preservation
Substances
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Cholic Acids
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Detergents
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Receptors, Angiotensin
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Receptors, Cell Surface
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Angiotensin II
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3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate