Abstract
Kirsten (Ki)-ras cDNA clones were prepared from human lung and colon carcinoma cell lines expressing an activated c-Ki-ras2 gene. DNA sequence analysis and transfection studies indicate that different point mutations at the same codon can activate the gene; that most human c-Ki-ras2 mRNA uses sequences from a fourth coding exon distinct from that of its viral counterpart; and that at least one cell line is functionally homozygous for the activated gene.
MeSH terms
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Alleles
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Cell Transformation, Neoplastic*
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Colonic Neoplasms / genetics*
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DNA, Neoplasm / genetics
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Gene Expression Regulation
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Humans
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Kirsten murine sarcoma virus / genetics*
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Lung Neoplasms / genetics*
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Mutation
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Oncogenes*
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RNA Splicing
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RNA, Neoplasm / genetics
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Sarcoma Viruses, Murine / genetics*
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Viral Proteins / genetics
Substances
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DNA, Neoplasm
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RNA, Neoplasm
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Viral Proteins
Associated data
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GENBANK/K00652
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GENBANK/K00653
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GENBANK/L00044
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GENBANK/L00045
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GENBANK/L00046
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GENBANK/L00047
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GENBANK/L00048
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GENBANK/L00049