Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations

Nature. 1983 Aug 11-17;304(5926):507-13. doi: 10.1038/304507a0.

Abstract

Kirsten (Ki)-ras cDNA clones were prepared from human lung and colon carcinoma cell lines expressing an activated c-Ki-ras2 gene. DNA sequence analysis and transfection studies indicate that different point mutations at the same codon can activate the gene; that most human c-Ki-ras2 mRNA uses sequences from a fourth coding exon distinct from that of its viral counterpart; and that at least one cell line is functionally homozygous for the activated gene.

MeSH terms

  • Alleles
  • Cell Transformation, Neoplastic*
  • Colonic Neoplasms / genetics*
  • DNA, Neoplasm / genetics
  • Gene Expression Regulation
  • Humans
  • Kirsten murine sarcoma virus / genetics*
  • Lung Neoplasms / genetics*
  • Mutation
  • Oncogenes*
  • RNA Splicing
  • RNA, Neoplasm / genetics
  • Sarcoma Viruses, Murine / genetics*
  • Viral Proteins / genetics

Substances

  • DNA, Neoplasm
  • RNA, Neoplasm
  • Viral Proteins

Associated data

  • GENBANK/K00652
  • GENBANK/K00653
  • GENBANK/L00044
  • GENBANK/L00045
  • GENBANK/L00046
  • GENBANK/L00047
  • GENBANK/L00048
  • GENBANK/L00049