Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture

Nature. 1983 Aug 18-24;304(5927):602-6. doi: 10.1038/304602a0.


The polyoma virus middle-T and the T24 Harvey ras1 genes are individually unable to transform primary baby rat kidney cells. Adenovirus early region 1A provides functions required by these genes to transform primary cells following DNA-mediated gene transfer. These results suggest that separate establishment and transforming functions are required for oncogenic transformation of primary cells in culture.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Antigens, Viral / genetics
  • Antigens, Viral, Tumor
  • Cell Transformation, Viral*
  • Cells, Cultured
  • Cloning, Molecular
  • Gene Expression Regulation
  • Genes, Viral*
  • Oncogenes*
  • Plasmids
  • Polyomavirus / genetics
  • Rats
  • Transfection
  • Transformation, Genetic


  • Antigens, Viral
  • Antigens, Viral, Tumor