Localization of c-ras oncogene family on human germ-line chromosomes

Proc Natl Acad Sci U S A. 1983 Aug;80(15):4794-7. doi: 10.1073/pnas.80.15.4794.

Abstract

The c-ras family is a set of c-onc genes that are highly conserved in vertebrates. The genes in this family are homologous to the transforming genes of Harvey and Kirsten murine sarcoma viruses (v-Ha-ras and v-Ki-ras, respectively). Using an in situ molecular hybridization method, we detected three sites on the human pachytene chromosomes that exhibited significant hybridization to v-Ki-ras and v-Ha-ras probes. These were chromomere positions that corresponded to bands 11p14.1, 12p12.1, and 12q24.2 of somatic chromosomes. The relationship between these chromosomal sites and previously defined members of the human c-ras gene family is discussed. These chromosomal sites are known to be involved in specific chromosome changes in a variety of tumors and in several congenital disorders that predispose to neoplastic disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoradiography
  • Carcinogens*
  • Chromosome Banding
  • Chromosomes, Human, 1-3 / ultrastructure*
  • Chromosomes, Human, 6-12 and X / ultrastructure*
  • Cloning, Molecular
  • DNA Transposable Elements
  • Humans
  • Nucleic Acid Hybridization
  • Tritium

Substances

  • Carcinogens
  • DNA Transposable Elements
  • Tritium