Abstract
A cytochemical method for 5'-nucleotidase localization in which cerium serves as the capture agent in order to enzymatically detect liberated inorganic phosphate has been developed. The method has been established in cell-free model systems and in guinea pig neutrophils where 5'-nucleotidase is restricted to the plasmalemma as an ectoenzyme. This cerium-based method gives better results for ultrastructural localization of 5'-nucleotidase than conventional lead-based methods.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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5'-Nucleotidase
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Adenosine Monophosphate / metabolism
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Alkaline Phosphatase / metabolism
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Animals
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Cerium* / metabolism
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Glycerophosphates / metabolism
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Guinea Pigs
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Histocytochemistry / methods*
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Male
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Models, Biological
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Neutrophils / enzymology
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Neutrophils / ultrastructure*
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Nitrophenols / metabolism
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Nucleotidases / blood*
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Organophosphorus Compounds / metabolism
Substances
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Glycerophosphates
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Nitrophenols
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Organophosphorus Compounds
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Cerium
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nitrophenylphosphate
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Adenosine Monophosphate
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Nucleotidases
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Alkaline Phosphatase
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5'-Nucleotidase
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beta-glycerophosphoric acid