A family of Saccharomyces cerevisiae repetitive autonomously replicating sequences that have very similar genomic environments

J Mol Biol. 1983 Aug 15;168(3):505-23. doi: 10.1016/s0022-2836(83)80299-x.

Abstract

We have characterized a family of moderately repetitive autonomously replicating sequences (ARSs) in Saccharomyces cerevisiae. Restriction mapping, deletion studies and hybridization studies suggest that these ARSs, which are probably less than 350 base-pairs in size, share one common feature: each is located close to, but not within, a repetitive sequence (131) of approximately 10(3) to approximately 1.5 X 10(3) base-pairs in length. These ARSs can be divided into two classes (X and Y) by their sequence homology and genomic environments. Each of the class X ARSs is embedded within a repetitive sequence (X) of variable length (approximately 0.3 X 10(3) to approximately 3.75 X 10(3) base-pairs); each of the class Y ARSs is embedded within a highly conserved repetitive sequence (Y) of approximately 5.2 X 10(3) base-pairs in length. Both of these sequences are located directly adjacent to the 131 sequence.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA Replication*
  • DNA Restriction Enzymes
  • DNA, Fungal / analysis
  • Nucleic Acid Hybridization
  • Repetitive Sequences, Nucleic Acid*
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic

Substances

  • DNA, Fungal
  • DNA Restriction Enzymes