One-step gene disruption in yeast

Methods Enzymol. 1983;101:202-11. doi: 10.1016/0076-6879(83)01015-0.

Abstract

The one-step gene disruption techniques described here are versatile in that a disruption can be made simply by the appropriate cloning experiment. The resultant chromosomal insertion is nonreverting and contains a genetically linked marker. Detailed knowledge of the restriction map of a fragment is not necessary. It is even possible to "probe" a fragment that is unmapped for genetic functions by constructing a series of insertions and testing each one for its phenotype.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Composition
  • Base Sequence
  • Cloning, Molecular*
  • DNA / analysis
  • DNA Restriction Enzymes
  • DNA, Fungal / genetics*
  • Genes*
  • Genetic Engineering / methods
  • Mutation
  • Plasmids
  • Saccharomyces cerevisiae / genetics*

Substances

  • DNA, Fungal
  • DNA
  • DNA Restriction Enzymes