Genetic applications of yeast transformation with linear and gapped plasmids

Methods Enzymol. 1983;101:228-45. doi: 10.1016/0076-6879(83)01017-4.

Abstract

Techniques for high frequency yeast transformation have been described. A double-strand break introduced by restriction enzyme cleavage can be used to direct a plasmid to integrate into a particular chromosomal locus. Plasmids containing a double-strand gap can be used in a straightforward method for the isolation and mapping of chromosomal alleles. These techniques extend the genetic applications of yeast transformation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Chromosomes / physiology
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics*
  • DNA, Fungal / genetics*
  • Escherichia coli / genetics*
  • Genetic Engineering / methods
  • Genetic Vectors*
  • Nucleic Acid Hybridization
  • Plasmids*
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*

Substances

  • DNA, Bacterial
  • DNA, Fungal
  • DNA Restriction Enzymes