Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function

Nucleic Acids Res. 1983 Sep 10;11(17):5855-64. doi: 10.1093/nar/11.17.5855.


A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA-Directed DNA Polymerase / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genes*
  • Genes, Bacterial*
  • Operon*
  • Structure-Activity Relationship
  • T-Phages / enzymology
  • Transcription, Genetic*


  • DNA-Directed DNA Polymerase
  • DNA Restriction Enzymes