The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis

Nucleic Acids Res. 1983 Sep 10;11(17):6089-105. doi: 10.1093/nar/11.17.6089.


Nucleotide sequences of the homologous tetracycline resistance (tet) determinants of plasmid RP1 and transposon Tn1721 have been determined. Two open reading frames of divergent polarity have been assigned to a regulatory gene (tetR) and a gene encoding a resistance protein (tetA). The intercistronic region contains appropriate regulatory and transcription signals. The tetR gene can code for a protein of 216 amino acids (deduced mol.wt. 23,288) and the tetA gene for a protein of 399 amino acids (deduced mol. wt. 42,205). Based on the deduced amino acid sequence, the tetA proteins of RP1/Tn1721 are 78% homologous with that of pBR322 and 45% homologous with that of Tn10. We conclude that a single tetA gene mediates resistance in each of these tet determinants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA Transposable Elements / drug effects*
  • Drug Resistance, Microbial
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Genes, Bacterial / drug effects*
  • Genes, Regulator / drug effects*
  • Plasmids / drug effects*
  • Tetracycline / pharmacology*
  • Transcription, Genetic


  • Bacterial Proteins
  • DNA Transposable Elements
  • DNA Restriction Enzymes
  • Tetracycline

Associated data

  • GENBANK/X00006