Effects of (E)-5-(2-bromovinyl)-2'-deoxyuridine on the proliferation of herpes simplex virus type 1-transformed and thymidine kinase-deficient mouse cells

Virology. 1983 Sep;129(2):490-2. doi: 10.1016/0042-6822(83)90188-5.

Abstract

(E)-5-(2-Bromovinyl)-2'-deoxyuridine (BVDU) is a highly selective inhibitor of herpes simplex virus-1 (HSV-1). A cell line (LH-1) expressing the HSV thymidine kinase (TK) gene is extremely sensitive to BVDU, whereas the parent line that expresses the cellular TK is resistant to BVDU. A TK-deficient derivative cell line (LMTK(-] which expresses neither the cellular nor viral TK was also extremely sensitive to BVDU. This suggests that the thymidine analog, BVDU, can be phosphorylated in thymidine kinase-deficient cells by pathways other than the cellular or HSV-encoded thymidine kinase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bromodeoxyuridine / analogs & derivatives*
  • Bromodeoxyuridine / pharmacology
  • Cell Division / drug effects*
  • Cell Line
  • Cell Transformation, Viral*
  • Dose-Response Relationship, Drug
  • Mice
  • Simplexvirus / enzymology
  • Simplexvirus / physiology*
  • Thymidine Kinase / deficiency
  • Thymidine Kinase / metabolism

Substances

  • brivudine
  • Thymidine Kinase
  • Bromodeoxyuridine