Pineal modulation of ACTH 1-17 effect upon murine corticosterone production

Brain Res Bull. 1983 Jul;11(1):117-25. doi: 10.1016/0361-9230(83)90060-6.


In tests of corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1-17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1-17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37 degrees C with 95% O2 and 5% CO2, the media were stored at -20 degrees C until corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1-17 of adrenal corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenal Glands / drug effects
  • Adrenal Glands / metabolism*
  • Adrenocorticotropic Hormone / pharmacology*
  • Animals
  • Circadian Rhythm
  • Corticosterone / biosynthesis*
  • Female
  • In Vitro Techniques
  • Kinetics
  • Mice
  • Mice, Inbred Strains
  • Peptide Fragments / pharmacology*
  • Pineal Gland / physiology*
  • Radioimmunoassay
  • Tissue Extracts / pharmacology


  • Peptide Fragments
  • Tissue Extracts
  • Adrenocorticotropic Hormone
  • ACTH (1-17)
  • Corticosterone