Identification of two distinct regulatory regions adjacent to the human beta-interferon gene

Cell. 1983 Oct;34(3):865-79. doi: 10.1016/0092-8674(83)90544-5.


To study the regulation of the human beta-interferon (beta-IFN) gene by poly(I)-poly(C), we analyzed the expression of deletion mutants of the cloned gene introduced into mouse cells on a new bovine papilloma virus (BPV) vector. In stable cell lines transformed by a BPV-IFN plasmid containing the beta-IFN structural gene with 210 bp of DNA to the 5' side of its mRNA cap site (denoted -210), human beta-IFN mRNA is induced approximately 400-fold by poly(I)-poly(C), and reproducible levels of expression are observed for independent cell lines. Our studies indicate that there are two distinct regulatory regions adjacent to the gene, located between -77 and -19, and between -210 and -107. The -77 to -19 region is required for constitutive and induced IFN gene expression, and both are drastically reduced by deletion to -73. When sequences between -210 and -107 are deleted, the constitutive level of IFN gene expression is increased 5- to 10-fold, while induced expression is essentially unaffected. Deletion of the -210 to -107 region also alters the kinetics of induction of the gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Bovine papillomavirus 1
  • Cell Transformation, Viral
  • Chromosome Deletion
  • DNA / analysis
  • Gene Expression Regulation* / drug effects
  • Humans
  • Interferon Type I / genetics*
  • Mutation
  • Poly I-C / pharmacology
  • RNA, Messenger / metabolism


  • Interferon Type I
  • RNA, Messenger
  • DNA
  • Poly I-C

Associated data

  • GENBANK/J00218
  • GENBANK/K00616
  • GENBANK/M11029