An IS15 insertion generates an eight-base-pair duplication of the target DNA

P Trieu-Cuot; A Labigne-Roussel; P Courvalin

doi:10.1016/0378-1119(83)90137-3

An IS15 insertion generates an eight-base-pair duplication of the target DNA

Gene. 1983 Sep;24(1):125-9. doi: 10.1016/0378-1119(83)90137-3.

Authors

P Trieu-Cuot, A Labigne-Roussel, P Courvalin

PMID: 6313479
DOI: 10.1016/0378-1119(83)90137-3

Abstract

In plasmid pIP1088 the transposable module IS15 is inserted at nucleotide position 1,430 of the vector plasmid pBR322. We have sequenced the termini of the IS15 element, which consists of two perfect inverted repeat sequences, 14 bp long. The sequence is 5'-GGCACTGTTGCAAA . . . TTTGCAACAGTGCC-3'. The integration event results in the duplication of 8 bp of target DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA Transposable Elements*
DNA, Bacterial / genetics*
Nucleic Acid Conformation
Recombination, Genetic*
Repetitive Sequences, Nucleic Acid

Substances

DNA Transposable Elements
DNA, Bacterial

Associated data

GENBANK/K01187
GENBANK/K01188