Abstract
Hybrid plasmid DNA cloned in Escherichia coli undergoes deletions when returned to competent Bacillus subtilis, even in defined restriction and modification mutants of strain 168. We have isolated a mutant of B. subtilis MI112 which is stably transformed at high frequency by chimeric plasmid DNA propagated in E. coli.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus subtilis / genetics*
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Bacillus subtilis / isolation & purification
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Cloning, Molecular*
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Escherichia coli / genetics*
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Genetic Vectors*
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Genotype
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Mutation*
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Nucleic Acid Hybridization
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Phenotype
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Plasmids*
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Species Specificity
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Transformation, Bacterial*