Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis: isolation of a spontaneous mutant of Bacillus subtilis with enhanced transformability for Escherichia coli-propagated chimeric plasmid DNA

J Bacteriol. 1983 Nov;156(2):934-6. doi: 10.1128/jb.156.2.934-936.1983.

Abstract

Hybrid plasmid DNA cloned in Escherichia coli undergoes deletions when returned to competent Bacillus subtilis, even in defined restriction and modification mutants of strain 168. We have isolated a mutant of B. subtilis MI112 which is stably transformed at high frequency by chimeric plasmid DNA propagated in E. coli.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacillus subtilis / genetics*
  • Bacillus subtilis / isolation & purification
  • Cloning, Molecular*
  • Escherichia coli / genetics*
  • Genetic Vectors*
  • Genotype
  • Mutation*
  • Nucleic Acid Hybridization
  • Phenotype
  • Plasmids*
  • Species Specificity
  • Transformation, Bacterial*