A third defective lambdoid prophage of Escherichia coli K12 defined by the lambda derivative, lambdaqin111

J Mol Biol. 1983 Nov 5;170(3):611-33. doi: 10.1016/s0022-2836(83)80124-7.

Abstract

We describe the isolation and characterization of a new Q-independent substitution mutant of lambda, lambdaqin111, which differs from other characterized Q-independent lambda phages. This mutant defines a new lambda-like prophage in the bacterial chromosome, as seen by homologous recombination between lambdaqin111 and the host DNA and by DNA/DNA hybridization methods. Genetic and electron microscopy data show that this new prophage carries, at least, genes analogous to Q-S-R of lambda and also a cos site functionally identical to lambda cos. It is located near 34 min on the Escherichia coli K12 map, i.e. in the same region but at a different site from the defective Rac prophage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / genetics*
  • Base Sequence
  • Chromosomes, Bacterial
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • DNA, Viral
  • Lysogeny*
  • Mutation*
  • Nucleic Acid Heteroduplexes
  • Plasmids
  • Virus Activation

Substances

  • DNA, Recombinant
  • DNA, Viral
  • Nucleic Acid Heteroduplexes
  • DNA Restriction Enzymes