Preparation and stabilization of aqueous/ethanolic superoxide solutions

Anal Biochem. 1983 Aug;133(1):170-8. doi: 10.1016/0003-2697(83)90239-7.

Abstract

Methods for the generation and stabilization of alkaline aqueous and/or ethanolic superoxide solutions have been developed utilizing either high energy ionizing radiation or vacuum-uv photolysis. These solutions can be used for the study of the reactivity of superoxide radical (O-2) and its conjugate acid the perhydroxyl radical (HO2) with other compounds over the entire pH range. Frozen, they can be kept at -196 degrees C for prolonged time periods with little loss in superoxide radical. As these solutions can be rendered free of molecular oxygen, studies of the reactivity of HO2/O-2 with oxygen-sensitive compounds under virtually anaerobic conditions are possible.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chemical Phenomena
  • Chemistry
  • Cold Temperature
  • Drug Stability
  • Edetic Acid
  • Ethanol
  • Microwaves
  • Photolysis
  • Solutions
  • Superoxides* / radiation effects
  • Water

Substances

  • Solutions
  • Water
  • Superoxides
  • Ethanol
  • Edetic Acid