Ethylisopropyl-amiloride: a new and highly potent derivative of amiloride for the inhibition of the Na+/H+ exchange system in various cell types

Biochem Biophys Res Commun. 1983 Oct 14;116(1):86-90. doi: 10.1016/0006-291x(83)90384-4.

Abstract

Ethylisopropyl-amiloride is 100 times more potent than amiloride for inhibiting the Na+/H+ exchanger of 3T3 fibroblasts, chick skeletal muscle cells and chick cardiac cells. Half-maximum effects, measured at 3 mM external Na+ are observed at 20-100 nM and 5 microM for ethylisopropyl-amiloride and amiloride respectively. As previously observed for amiloride, the effect of ethylisopropyl-amiloride is antagonized by external Na+ ions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amiloride / analogs & derivatives
  • Amiloride / pharmacology*
  • Animals
  • Carrier Proteins / antagonists & inhibitors*
  • Cells, Cultured
  • Chickens
  • Dose-Response Relationship, Drug
  • Mice
  • Pyrazines / pharmacology*
  • Sodium-Hydrogen Exchangers
  • Structure-Activity Relationship

Substances

  • Carrier Proteins
  • Pyrazines
  • Sodium-Hydrogen Exchangers
  • Amiloride
  • ethylisopropylamiloride