Characterization of a Y/15 translocation by banding methods, distamycin A treatment of lymphocytes and DNA restriction endonuclease analysis

Clin Genet. 1983 Oct;24(4):234-9. doi: 10.1111/j.1399-0004.1983.tb00076.x.

Abstract

A de novo 14/21 Robertsonian translocation and a familially inherited Y/15 translocation were observed in a male infant with anomalies of the external genitalia. The Y/15 translocation was confirmed by cultivating lymphocytes in a medium containing distamycin A and by determining the occurrence of the Y-specific DNA sequences by means of Hae III restriction endonuclease analysis. Any connection between the structural chromosomal abnormalities and the symptoms of the infant is highly improbable.

Publication types

  • Case Reports

MeSH terms

  • Chromosome Banding
  • Chromosomes, Human, 13-15*
  • Chromosomes, Human, 21-22 and Y
  • DNA / genetics
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific*
  • Distamycins / pharmacology
  • Humans
  • Hypospadias / genetics*
  • Infant
  • Lymphocytes / drug effects
  • Male
  • Translocation, Genetic*
  • Y Chromosome*

Substances

  • Distamycins
  • stallimycin
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific
  • GGCC-specific type II deoxyribonucleases