Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion

H Schweizer; W Boos

doi:10.1007/BF00327683

Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion

Mol Gen Genet. 1983;192(1-2):293-4. doi: 10.1007/BF00327683.

Authors

H Schweizer, W Boos

PMID: 6316111
DOI: 10.1007/BF00327683

Abstract

To facilitate construction of mutants harboring delta lac for use in gene fusion studies, strains were constructed that carry the transposon Tn10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Deletion
DNA Transposable Elements*
Escherichia coli / genetics*
Genes, Bacterial*
Lac Operon
Mutation

Substances

DNA Transposable Elements