Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations

Nature. 1983 Dec 15-21;306(5944):658-61. doi: 10.1038/306658a0.


Each of nine mammary carcinomas induced by a single injection of nitroso-methylurea into 50-day-old Buf/N female rats, contained a transforming H-ras-1 gene. Molecular characterization of one of the genes revealed that the twelfth codon was GAA instead of GGA of the normal allele, encoding glutamic acid in place of glycine. These results indicate that chemical carcinogenesis represents an adequate model to study the role of transforming ras genes in human neoplasia.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Restriction Enzymes
  • Female
  • Mammary Neoplasms, Experimental / chemically induced
  • Mammary Neoplasms, Experimental / genetics*
  • Methylnitrosourea / toxicity*
  • Mutation*
  • Neoplasm Proteins / genetics
  • Nitrosourea Compounds / toxicity*
  • Oncogenes / drug effects*
  • Rats


  • Neoplasm Proteins
  • Nitrosourea Compounds
  • Methylnitrosourea
  • DNA Restriction Enzymes