Characterization and visualization of cholecystokinin receptors in rat brain using [3H]pentagastrin

Peptides. 1983 Sep-Oct;4(5):755-62. doi: 10.1016/0196-9781(83)90032-3.


[3H]Pentagastrin binds specifically to an apparent single class of CCK receptors on slide-mounted sections of rat brain (KD = 5.6 nM; Bmax = 36.6 fmol/mg protein). This specific binding is temperature-dependent and regulated by ions and nucleotides. The relative potencies of C-terminal fragments of CCK-8(SO3H), benzotript and proglumide in inhibiting specific [3H]pentagastrin binding to CCK brain receptors reinforce the concept of different brain and pancreas CCK receptors. CCK receptors were visualized by using tritium-sensitive LKB film analyzed by computerized densitometry. CCK receptors are highly concentrated in the cortex, dentate gyrus, granular and external plexiform layers of the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, claustrum, accumbens nucleus, some nuclei of the amygdala, thalamus and hypothalamus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Binding, Competitive
  • Brain / metabolism*
  • Cholecystokinin / analogs & derivatives
  • Cholecystokinin / metabolism*
  • Cholecystokinin / pharmacology
  • Corpus Striatum / metabolism
  • Guanine Nucleotides / pharmacology
  • Kinetics
  • Male
  • Pentagastrin / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Cell Surface / drug effects
  • Receptors, Cell Surface / metabolism*
  • Receptors, Cholecystokinin
  • Tritium


  • Guanine Nucleotides
  • Receptors, Cell Surface
  • Receptors, Cholecystokinin
  • Tritium
  • Cholecystokinin
  • Pentagastrin