Expression of human alpha 1-antitrypsin in Escherichia coli

FEBS Lett. 1984 Jan 23;166(1):67-70. doi: 10.1016/0014-5793(84)80046-0.

Abstract

Complementary DNA coding for human alpha 1-antitrypsin has been placed under the control of the lambda PR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human alpha 1-antitrypsin (+/- 9000 molecules/cell). The product has a Mr of 44000, corresponding to mature unglycosylated alpha 1-antitrypsin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / genetics
  • Gene Expression Regulation
  • Genetic Vectors
  • Glycoproteins / genetics
  • Humans
  • Operon
  • Plasmids
  • alpha 1-Antitrypsin / genetics*

Substances

  • Glycoproteins
  • alpha 1-Antitrypsin