Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae

Gene. 1983 Nov;25(2-3):179-88. doi: 10.1016/0378-1119(83)90223-8.

Abstract

The plasmid-borne gene hph coding for hygromycin B phosphotransferase (HPH) in Escherichia coli has been identified and its nucleotide sequence determined. The hph gene is 1026 nucleotides long, coding for a protein with a predicted Mr of 39 000. The hph gene was placed in a shuttle plasmid vector, downstream from the promoter region of the cyc 1 gene of Saccharomyces cerevisiae, and an hph construction containing a single AUG in the 5' noncoding region allowed direct selection following transformation in yeast and in E. coli. Thus the hph gene can be used in cloning vectors for both pro- and eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Cloning, Molecular
  • Culture Media
  • Drug Resistance, Microbial
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression Regulation
  • Hygromycin B / pharmacology*
  • Kanamycin Kinase
  • Phosphotransferases / genetics*
  • Plasmids*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • Hygromycin B
  • Phosphotransferases
  • Kanamycin Kinase

Associated data

  • GENBANK/K01193