Isolation of a human prostate carcinoma cell line (DU 145)

Int J Cancer. 1978 Mar 15;21(3):274-81. doi: 10.1002/ijc.2910210305.


A long-term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acid Phosphatase / metabolism
  • Adenocarcinoma* / genetics
  • Adenocarcinoma* / ultrastructure
  • Aged
  • Aneuploidy
  • Brain / ultrastructure
  • Brain Neoplasms / genetics
  • Brain Neoplasms / pathology
  • Cell Line*
  • Chromosome Aberrations
  • Culture Techniques
  • Humans
  • Male
  • Neoplasm Metastasis
  • Prostatic Neoplasms* / genetics
  • Prostatic Neoplasms* / ultrastructure


  • Acid Phosphatase