Entry of Mouse Hepatitis Virus 3 Into Cells

J Gen Virol. 1984 Jan;65 ( Pt 1):227-31. doi: 10.1099/0022-1317-65-1-227.


The involvement of lysosomes in infection by mouse hepatitis virus 3 (MHV3) was studied. L cells were infected with MHV3 in the presence of NH4Cl or chloroquine, weak bases which increase the intralysosomal pH and impair lysosomal functions. NH4Cl significantly inhibited virus-induced cytopathic effects and MHV3 replication, but did not prevent the attachment of 3H-labelled virus. No inhibition of MHV3 replication by NH4Cl and chloroquine was observed when lysosomotropic agents were added later than 3 h post-infection, suggesting the direct involvement of lysosomes in release of the viral genome into cytoplasm. These results, together with the lack of antibody-mediated immune lysis of MHV3-infected cells, suggest that MHV3 entered cells by an endocytic pathway (viropexis) followed by internalization into cellular lysosomes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ammonium Chloride / pharmacology
  • Animals
  • Chloroquine / pharmacology
  • Cytopathogenic Effect, Viral
  • Hepatitis, Viral, Animal / microbiology*
  • L Cells / drug effects
  • L Cells / microbiology
  • Lysosomes / drug effects
  • Lysosomes / microbiology
  • Mice
  • Murine hepatitis virus / drug effects
  • Murine hepatitis virus / pathogenicity
  • Virus Replication / drug effects


  • Ammonium Chloride
  • Chloroquine